Three Dimensional Cell Culture
| Site: | clevaforce |
| Course: | 3D Cell Culture |
| Book: | Three Dimensional Cell Culture |
| Printed by: | Guest user |
| Date: | Thursday, 28 November 2024, 10:44 AM |
Description
A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments such as the Petri dish, a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in living systems.
These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies.
1. Cell Culture: 2D vs 3D
Cell culture is a research technique where cells are removed from an organism and grown in suitable artificial conditions.
Cell culture is generally of two types with each with its own set of advantages and disadvantages.
2. 3D Cell Culture
3D cell culture is the cultivation of living cells within micro-assembled devices and supports with three-dimensional structures that mimic tissue and organ-specific microarchitecture.
The use of 3D culture is an accurate method of reproducing this process in vitro. 3D cell culture is becoming more popular due to its novel and convenient features when compared to other cell culture methods.
3. Types of 3D Cell Culture - Scaffold 3D Cell Culture
Scaffold based 3D Cell Culture use hydrogels or structural scaffolds to ensure maturing cells interact with one another and eventually form structures that are similar to the tissues from which they originated.
4. Types of 3D Cell Culture - Scaffold Free 3D Cell Culture
Scaffold-free cell culture approaches use freely floating cell aggregates, typically referred to as spheroids.
5. Applications of 3D Cell Culture
There are many applications of 3D including but not limited to Tissue Engineering, Organ-on-Chip and Drug Testing