A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments such as the Petri dish, a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in living systems.
These three-dimensional cultures are usually grown in bioreactors, small capsules in which the cells can grow into spheroids, or 3D cell colonies.
Cell culture is a research technique where cells are removed from an organism and grown in suitable artificial conditions.
Cell culture is generally of two types with each with its own set of advantages and disadvantages.